Autosamplers are special programmable devices for automatic input of samples into the device. The use of the device significantly reduces the time of analysis, especially in case of a large flow of studies and eliminates the influence of a human factor. The combination of different algorithms of input, temperature modes, dosing of autosampler allows to increase the reliability of results depending on the properties of samples.
Autosamplers are characterized by ease of use and high performance. The devices provide rapid analysis within one working cycle. Most models of devices can operate in 3 modes: full, partial filling, as well as selection by microliters. The work process includes three stages:
Installation of tubes in the carousel of the autosampler.
Automated movement of the probe and sample collection.
Moving the probe to the flush port where the system is flushed to eliminate cross-contamination.
The autosampler is controlled by software. The device is easily connected to a computer. The device is often used to input samples into chromatographs of different series. This combination allows the equipment to be controlled by the program installed in the chromatograph.
The autosampler carousel consists of removable racks in which sample tubes of the required volume are placed. The software automatically recognizes the installed racks. The technician independently sets the programs for sample introduction and syringe flushing. If an autosampler is purchased for a laboratory, employees must be trained step-by-step before starting work. First, it is important to learn the functions, then the settings, and then move on to practice.
Autosamplers produce different models, which differ in technical characteristics and features of sampling. There are three main types of devices:
with filling the loop by vacuuming;
with loop filling by injection;
with built-in loop dispenser.
The first type involves filling the injector's loop dispenser with a sample by negative pressure generated by a syringe. The second brings the syringe to the sample vial, withdraws a predetermined volume, and injects it into the tap port, filling the loop. The processes are controlled by a stepper motor. Autosamplers with a built-in loop allow automatic sample introduction without loss.
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