HPLC stands for high-performance liquid chromatography, a technique used in analytical chemistry for the separation, detection, and determination of components in a mixture. To implement this technique, it is important to purchase specialized HPLC pumps for the laboratory to ensure high research efficiency. Typically, these devices are used in combination with a column filled with a solid adsorbing material. A sample is passed through the column under pressure, resulting in the separation of substances. The devices are also utilized in other applications, such as dosing reagents.
HPLC pumps are available at favorable prices on the Bimedis website and are widely used in various applications:
production of pharmaceutical and biological products;
detection of specific compounds in urine;
separation of complex sample components, synthetic substances with similar properties;
determination of the amount of vitamin D in blood serum;
various kinds of research.
The devices are important in medicine, law, biology, and chemistry. They accurately dose and continuously feed solvent into the chromatographic column under pressures of several tens of MPa. Microcolumn liquid chromatography, especially capillary chromatography, requires pumps with a capacity of 1 µL per minute, while preparative chromatography requires a capacity of 25 to 100 µL.
HPLC pumps are available in different models, which affects the cost of the equipment. When choosing a device, the technical characteristics should meet the laboratory's requirements. First and foremost, it is important to consider the materials from which the device is made; they must be resistant to the effects of chemicals. Quality models are made from stainless alloys and have fluoroplastic or polyamide sealing components. The main criteria that are important when selecting an HPLC pump include:
type: piston or syringe;
sufficient working pressure;
level of flow stability;
flexibility in mixing solvents for gradient elution;
compatibility with different solvents;
backpressure range.
To avoid damage to the system during high-performance liquid chromatography, it is important to determine which types of solvents and mixtures will be used most frequently. A compatible pump should then be selected. The amount of backpressure in the instrument depends on the type of column used.
Bimedis Marketplace offers reasonable prices for HPLC pumps and other medical and laboratory equipment and products. The marketplace unites buyers and sellers around the world with favorable deals. To place an order, you need to select the model of the device that has the right specifications. Experienced consultants will help with the selection, provide advice and answers to questions.